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Instituto de Biotecnología y Biología Molecular (IBBM),
Universidad Nacional de La Plata - CONICET, Calle 115 entre 49 y
50, La Plata, Argentina
ibbm@biol.unlp.edu.ar
Sponsored by CABBIO, Centro Argentino Brasilero de Biotecnología
Directores: Drs. O. Mario Aguilar y Flavio A. Blanco
Objetivos/Objectives:
Con un enfoque crítico, proveer información actualizada sobre el
uso de herramientas basadas en la genómica de leguminosas para
su aplicación en el avance del conocimiento en la región, y en la
solución de problemas y limitaciones de la agricultura en su forma
más amplia y comprensiva.
Temáticamente, el curso abordará aspectos de la genómica funcional
considerándola un método científico que busca identificar y
definir la función de los genes y descubrir cuándo y cómo trabajan
juntos los genes para producir caracteres. En especial, se
desarrollarán los resultados de investigaciones recientes sobre la
identificación de factores genéticos de leguminosas participantes
en el proceso de interacción simbiótica con rizobios, la
identificación de mecanismos nuevos de regulación basado en miRNAs,
y la transformación génica de leguminosas.
TEMAS/TOPICS
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Legumes, their significance as cropping and model plants for
basic research on symbiosis with rhizobia
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Transcriptional factor in legumes. Structure, families. General
and specific functions. Transduction pathways. Approaches to
determine functions.
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Genetic transformation of leguminous plants.
Heterologous gene expression and silencing in leguminous species.
Composite plants by using Agobacterium rhizogenes; reporter
genes, and RNAi strategies to investigate symbiotic functions.
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Evolution of symbiotic signaling in Legumes. Perception of
rhizobial Nodulation (Nod) factors and subsequent induction of
symbiotic responses is a unique legume feature. Signal
transduction.
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Hypotheses on the evolution of symbiotic signaling and the
methodologies used to test them.
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Actinorhizal genomics : the establishment of ESTs collections
and microrrays for two actinorhizal trees ; Casuarina and Alnus
and the comparative analysis of root, nodule and mycorhizal
transcriptomes. Recent findings on Frankia genomics including
the sequencing of several Frankia genomes and the analysis of
the Frankia transcriptome RNAi and complementation strategies
that unveiled common genetic mechanisms shared by rhizobial,
actinorhizal and mycorrhizal symbiosis.
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Regulation of root architecture and symbiosis in legumes :
comparison of lateral root development and nodule development.
Impact of abiotic stresses on root growth in legumes, functional
analysis of transcriptome results, transcription factors and
receptor kinases.
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Regulatory RNAs and the control of developmental plasticity
(non-coding RNAs in development and stress responses, small RNAs,
silencing and RNAi, deep sequencing of small RNAs). Analysis of
small RNA diversity in Legumes, the dynamic transcriptome
interactions between transcription factors and miRNAs.
Students will be asked to
present a seminar on their research project during the first week
of the course. Literature seminars on selected papers will be
prepared and presented by groups of students during the second
week of the course.
Profesores/Lecturers
IBBM
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Aguilar, O. Mario
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Battaglia, Marina
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Blanco, Flavio A.
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Zanetti, María Eugenia
Invited Lecturers
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Aragao, Francisco, EMBRAPA-Brasilia, Brazil
- Borsani,
Julio Omar, Universidad de la República, Montevideo, Uruguay
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**Crespi, Martin, CNRS, Gif sur Yvette, France
- Geurts, Renè, Wageningen University, The Netherlands
- **Svistoonoff,
Sergio, IRD, Montpellier, France
**Apoyo del
Programa cooperación Francia Ecos Sud
Asistentes/Instructors
- Beker,
Maria Pia, IBBM, La Plata, Argentina
- Flores, Ana
Claudia, IBBM, La Plata, Argentina
Alumnos/Students
Alumnos/Students
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Ricardo Weber (Brasil) -
rinweber@yahoo.com.br
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Paula Oblessuc (Brasil) -
parobl@gmail.br
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Carla Quinhones (Brasil) -
carlaquinhones@yahoo.com.br
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Karim Luchmann (Brasil) -
khluchmann@yahoo.com.br
- Roberto
Sierra (Colombia) -
robersm@gmail.com
- Ana Paula
Mulet (R.O. del Uruguay) -
anapaulamulet@gmail.com
- Manuel Muñoz (Chile) -
manuelmun@gmail.com
- Mauricio A. Reynoso (La Plata, Argentina)
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mauricioreynoso@gmail.com
- German Robert (Córdoba, Argentina) -
grobert@correo.inta.gov.ar
- Nacira Muñoz (Córdoba, Argentina) -
naciramunoz@yahoo.com
- Vivien Pentreath (Comodoro Rivadavia, Argentina)
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vivienpentreath@yahoo.com.ar
- María de los Angeles Giusti (La Plata,
Argentina) -
giusti@biol.unlp.edu.ar
- Virginia Dalla Via
(La Plata, Argentina) -
virdallavia@hotmail.com
Contacto:
O.
Mario Aguilar E-mail:
aguilar@biol.unlp.edu.ar
Cronograma |
